Preparation of Lipid-Conjugated siRNA Oligonucleotides for Enhanced Gene Inhibition in Mammalian Cells

Nucleic acid conjugates are promising drugs for treating gene-related diseases. Conjugating specific units like lipids, cell-penetrating peptides, polymers, antibodies, and aptamers either at the 3'- or 5'-termini of a siRNA duplex molecule has resulted in plethora bioconjugates with improved stabilities bloodstream better pharmacokinetic values than unmodified siRNAs. In this sense, lipid-siRNA have attracted remarkable interest their potential value facilitating cellular uptake. chapter, we describe series protocols involving synthesis oligonucleotides carrying neutral cationic lipids 5'-termini. The resulting aimed to be used as exogenous effectors inhibiting gene expression by RNA interference. A protocol formulation lipid using sonication presence serum is described yielding interesting transfection properties cell culture without use transfecting agents.